β-glucosidase B (BglB, lovingly called "Bagel B" by our teams) is an enzyme that catalyzes the hydrolysis of glucose monosaccharides from larger molecules at a β-glycosidic linkage. It is an essential enzyme for the degradation of cellulose by bacteria and fungi.
Our computational designs and kinetic measurements will utilize para-nitrophenyl-β-d-glucopyranose (pNPG) as a colorimetric reporter substrate.