About β-glucosidase B
Species:Paenibacillus polymyxa
EC Number:3.2.1.21
UniProt Number:P22505
PCB Entries:2JIE 2O9P 2O9R 2O9T 2Z1S
Molar Mass:51,573 Da
Extinction Coefficient (εBglB):113,330 m−1 cm−1
β-glucosidase B (BglB, lovingly called "Bagel B" by our teams) is an enzyme that catalyzes the hydrolysis of glucose monosaccharides from larger molecules at a β-glycosidic linkage. It is an essential enzyme for the degradation of cellulose by bacteria and fungi.
Our computational designs and kinetic measurements will utilize para-nitrophenyl-β-d-glucopyranose (pNPG) as a colorimetric reporter substrate.
Full BglB Sequence
One-letter amino acid residue codes in plain type (not bold) were not resolved in the crystal structure used for our design study (PDB #2JIE). One-letter codes in blue have parameter data stored in our database. Hovering over any 1-letter code in the sequence will give that residues sequence numbers/positions.